无透镜全息显微细胞成像

王雪; 刘虹遥; 路鑫超; 孙旭晴; 叶一霏; 黄成军

doi:10.3788/OPE.20202808.1644

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无透镜全息显微细胞成像

现代应用光学 | 更新时间：2020-08-14

- 无透镜全息显微细胞成像
- Cell imaging by holographic lens-free microscopy
- 光学精密工程 2020年28卷第8期页码：1644-1650
- 作者机构：
  
  1.中国科学院微电子研究所, 北京 100029
  2.中国科学院大学, 北京 100049
- 作者简介：
  
  [ "王雪(1991-), 男, 河南商丘人, 博士研究生, 2015年于青岛大学获得学士学位, 2018年于首都师范大学获得硕士学位, 主要从事无透镜成像和表面等离子体激元成像方面的研究。E-mail:wangxue@ime.ac.cn" ]
  路鑫超(1977-), 女, 副研究员, 2000年于天津大学获得学士学位, 2003年于天津大学获得硕士学位, 2009年于美国oklahoma州立大学获得博士学位, 现任职于中国科学院微电子研究所健康电子中心, 主要从事表面等离激元成像、微纳传感研究。E-mail:luxinchao@ime.ac.cnLU Xin-chao, E-mail:luxinchao@ime.ac.cn
  黄成军(1979-), 男, 研究员, 博士生导师, 2001年, 2006年于华中科技大学分别获得学士、博士学位, 现为中国科学院微电子研究所健康电子研发中心主任, 主要研究方向为微纳传感器、执行器与系统; MEMS器件的设计、仿真与工艺; 微流控芯片技术与芯片实验室系统。E-mail:huangchengjun@ime.ac.cnHUANG Cheng-jun, E-mail:huangchengjun@ime.ac.cn
- 基金信息：
  
  国家重点研发计划资助项目(2018YFC2001100);北京市自然科学基金资助项目(4192063);北京市自然科学基金资助项目(4182073)
- DOI：10.3788/OPE.20202808.1644
  中图分类号： O436.1;Q336
- 收稿日期：2020-01-09，
  
  修回日期：2020-02-26，
  
  录用日期：2020-2-26，
  
  纸质出版日期：2020-08-15
- 稿件说明：
移动端阅览
王雪, 刘虹遥, 路鑫超, 等. 无透镜全息显微细胞成像[J]. 光学精密工程, 2020,28(8):1644-1650.

Xue WANG, Hong-yao LIU, Xin-chao LU, et al. Cell imaging by holographic lens-free microscopy[J]. Optics and precision engineering, 2020, 28(8): 1644-1650.
王雪, 刘虹遥, 路鑫超, 等. 无透镜全息显微细胞成像[J]. 光学精密工程, 2020,28(8):1644-1650. DOI： 10.3788/OPE.20202808.1644.

Xue WANG, Hong-yao LIU, Xin-chao LU, et al. Cell imaging by holographic lens-free microscopy[J]. Optics and precision engineering, 2020, 28(8): 1644-1650. DOI： 10.3788/OPE.20202808.1644.

摘要

为了同时满足较大的视场和较高分辨率的需求，开发了一套全息无透镜显微成像系统和配套算法，实现对微米级样品的无透镜显微成像。搭建了一套由LED光源、针孔、被测样品与CMOS图像传感器组成的全息无透镜显微成像系统，并对针孔直径、成像面尺寸、光源到样品的距离，以及样品面到CMOS图像传感器的距离进行了优化。其次，开发了从系统采集的全息图中恢复样品图像的角谱法算法。最后，使用该成像系统和配套算法，分别对具有微米级结构分辨率测试靶，和肺癌细胞悬浮液进行了显微成像。该全息无透镜显微成像系统的分辨率为4.4

m，成像视场尺寸为5.7 mm×4.3 mm，实现了微米级结构和肺癌细胞较清晰的显微成像。全息无透镜显微成像系统结构简单、无像差干扰，可以实现大视场下较高分辨率的显微成像。

Abstract

This study develops a lens-free microscope system and an algorithm to realize microscopic imaging with a relatively large field of view and high resolution. The proposed system consists of an LED light source

a pinhole

a sample holder

and a CMOS imager. The system parameters

such as the pinhole diameter

size of the imager

distance between the LED and sample

and distance between the sample and imager were studied and optimized. Furthermore

an angular spectrum method was developed for recovering sample images from holograms captured by the CMOS imager.Finally

the developed system was employed to demonstrate lens-free microscopic imaging of a micropatterned testing chip with a standard resolution in addition to that of lung cancer cells in suspension.The developed holographic lens-free microscopic system has a resolution of 4.4

m and an imaging field of view up to 5.7 mm×4.3 mm; furthermore

it is capable of imaging the micropatterns in the test chip as well as individual lung cancer cells.Thus

the proposed holographic lens-free microscopic system demonstrated microscopic imaging with a relatively large field of view and high resolution; moreover

it exhibited the advantages of a simple structure and the avoidance of aberration interference.

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references

H M OZAKTAS , H UREY . Space-bandwidth product of conventional Fourier transforming systems . Optics Communications , 1993 . 104 ( 1/2/3 ): 29 - 31 . http://cn.bing.com/academic/profile?id=493d6a3880d4cc75aefb87f0b2192d16&encoded=0&v=paper_preview&mkt=zh-cn http://cn.bing.com/academic/profile?id=493d6a3880d4cc75aefb87f0b2192d16&encoded=0&v=paper_preview&mkt=zh-cn .

丁汛 , 赵跃进 , 丁玉奎 . 基于多图像融合的MEMS显微三维形貌重构 . 光学精密工程 , 2018 . 26 ( 5 ): 1275 - 1285 . http://ope.lightpublishing.cn/thesisDetails?columnId=1415824&Fpath=&index=-1&l=zh http://ope.lightpublishing.cn/thesisDetails?columnId=1415824&Fpath=&index=-1&l=zh .

X DING , Y J ZHAO , Y K DING . Three-dimensional microscopic reconstruction of MEMS based on multi image fusion . Opt. Precision Eng. , 2018 . 26 ( 5 ): 1275 - 1285 . http://ope.lightpublishing.cn/thesisDetails?columnId=1415824&Fpath=&index=-1&l=zh http://ope.lightpublishing.cn/thesisDetails?columnId=1415824&Fpath=&index=-1&l=zh .

王勇 , 王宇庆 , 马娇 . 改进的响尾蛇双模式细胞模型的图像融合 . 光学精密工程 , 2016 . 24 ( 11 ): 2848 - 2854 . http://ope.lightpublishing.cn/thesisDetails?columnId=1405839&Fpath=&index=-1&l=zh http://ope.lightpublishing.cn/thesisDetails?columnId=1405839&Fpath=&index=-1&l=zh .

Y WANG , Y Q WANG , J MA . Improved rattle snake dual mode cell model for image fusion . Opt. Precision Eng. , 2016 . 24 ( 11 ): 2848 - 2854 . http://ope.lightpublishing.cn/thesisDetails?columnId=1405839&Fpath=&index=-1&l=zh http://ope.lightpublishing.cn/thesisDetails?columnId=1405839&Fpath=&index=-1&l=zh .

A GREENBAUM , W LUO , T W SU , 等 . Imaging without lenses:achievements and remaining challenges of wide-field on-chip microscopy . Nature Methods , 2012 . 9 ( 9 ): 889 - 895 . DOI: 10.1038/nmeth.2114 http://doi.org/10.1038/nmeth.2114 .

李建素 , 党长营 . 预放大数字全息系统记录距离的设计 . 光学精密工程 , 2019 . 27 ( 4 ): 784 - 794 . http://ope.lightpublishing.cn/thesisDetails?columnId=1426173&Fpath=&index=-1&l=zh http://ope.lightpublishing.cn/thesisDetails?columnId=1426173&Fpath=&index=-1&l=zh .

J S LI , CH Y DANG . Design of recording distance in pre-magnification digital holography . Opt. Precision Eng. , 2019 . 27 ( 4 ): 784 - 794 . http://ope.lightpublishing.cn/thesisDetails?columnId=1426173&Fpath=&index=-1&l=zh http://ope.lightpublishing.cn/thesisDetails?columnId=1426173&Fpath=&index=-1&l=zh .

A OZCAN , E MCLEOD . Lensless imaging and sensing . Annual Review of Biomedical Engineering , 2016 . 18 ( 1 ): 77 - 102 . http://cn.bing.com/academic/profile?id=211ac4b4e6506e31b4d5240fb6403974&encoded=0&v=paper_preview&mkt=zh-cn http://cn.bing.com/academic/profile?id=211ac4b4e6506e31b4d5240fb6403974&encoded=0&v=paper_preview&mkt=zh-cn .

E MCLEOD , A OZCAN . Microscopy without lenses . Physics Today , 2017 . 70 ( 9 ): 50 - 56 . DOI: 10.1063/PT.3.3693 http://doi.org/10.1063/PT.3.3693 .

D LANGE , C W STORMENT , C A CONLEY , 等 . A microfluidic shadow imaging system for the study of the nematode Caenorhabditis elegans in space . Sensors and Actuators B:Chemical , 2005 . 107 ( 2 ): 904 - 914 . http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=d663227591a622820f00369b471661b2 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=d663227591a622820f00369b471661b2 .

A F COSKUN , T W SU , I SENCAN , 等 . Lensless fluorescent microscopy on a chip . Journal of Visualized Experiments , 2011 . ( 54 ): DOI: 10.3791/3181 http://doi.org/10.3791/3181 .

M SANZ , J A PICAZOBUENO , L GRANERO , 等 . Compact, cost-effective and field-portable microscope prototype based on MISHELF microscopy . Scientific Reports , 2017 . 7 ( 1 ): 43291 DOI: 10.1038/srep43291 http://doi.org/10.1038/srep43291 .

R W GERCHBERG . A practical algorithm for the determination of phase from image and diffraction plane pictures . Optik , 1972 . 35 ( 2 ): 237 - 246 . http://cn.bing.com/academic/profile?id=520585bcc1c7576dde8a0e405f344960&encoded=0&v=paper_preview&mkt=zh-cn http://cn.bing.com/academic/profile?id=520585bcc1c7576dde8a0e405f344960&encoded=0&v=paper_preview&mkt=zh-cn .

J R FIENUP . Phase retrieval algorithms:a comparison . Applied Optics , 1982 . 21 ( 15 ): 2758 - 2769 . DOI: 10.1364/AO.21.002758 http://doi.org/10.1364/AO.21.002758 .

A GREENBAUM , A OZCAN . Maskless imaging of dense samples using pixel super-resolution based multi-height lensfree on-chip microscopy . Optics Express , 2012 . 20 ( 3 ): 3129 - 3143 . http://cn.bing.com/academic/profile?id=6c6068b6381b631489fb1e93ab45587d&encoded=0&v=paper_preview&mkt=zh-cn http://cn.bing.com/academic/profile?id=6c6068b6381b631489fb1e93ab45587d&encoded=0&v=paper_preview&mkt=zh-cn .

Z GÖRÖCS , M TAMAMITSU , V BIANCO , 等 . A deep learning-enabled portable imaging flow cytometer for cost-effective, high-throughput, and label-free analysis of natural water samples . Light-Science & Applications , 2018 . 7 ( 1 ): 66 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=gkxyyy-e201805031 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=gkxyyy-e201805031 .

GOODMAN J W. Digital image formation from electronically detected holograms[C]. Proc SPIE 0010, Computerized Imaging Techniques , 1967, 0010: 176-181.

A S OSTROVSKY , G MARTíNEZ-NICONOFF , P MARTíNEZ-VARA , 等 . The van Cittert-Zernike theorem for electromagnetic fields . Optics Express , 2009 . 17 ( 3 ): 1746 - 1752 . http://cn.bing.com/academic/profile?id=c34c51be279abc86a64f514afe939430&encoded=0&v=paper_preview&mkt=zh-cn http://cn.bing.com/academic/profile?id=c34c51be279abc86a64f514afe939430&encoded=0&v=paper_preview&mkt=zh-cn .

J W GOODMAN . Introduction To Fourier Optics , McGraw-Hill , 1995 .

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